optical microscope

How do I use an optical microscope?  

1. Place the microscope in a bright place with direct sunlight.  

2. Turn the thick handle to remove the revolver from the stage.  

3. Remove the lens from the housing.  Since the objective lens is stored with the mounting part facing up, to prevent dust from invading the mounting part, the main body of the housing is inserted and removed.  

4. Goggles and objective lenses are used to prevent dust from entering the lens tube.  Rotate revolver to select low power objective.  

5. Move the mirror through the eyepiece to make the brightness even.  Do not use direct sunlight, as this is dangerous.  

6. Place the preparation work on the platform and fix it so that the object is directly under the objective lens.  

7. When looking at the microscope from the side, move the thick handle so that the objective and slide are close together.  

8. When looking through the eyepiece, use the thick handle to move the objective lens away from the stage and focus.  At this point, if rotated in the opposite direction, it is possible to make the preparation and objective contact each other and damage them.  

9. When the subject is clearly in focus, move the slide to find images that are easy to see.  The image is displayed upside down, left to right.  

10. If necessary, rotate the revolver to replace it with a high magnification objective.  Typically, it’s designed to have the same focus, so you won’t lose focus even if you change the objective lens.  

11. Observe the illuminance of the aperture and adjust the focus using the trimmer handle.  

12. Wipe off dust after use.  If it is used frequently, the lens is not always removed but is usually removed and stored in the opposite installation order.  If the lens is dirty, follow the instructions to clean it.  Usually, as with camera lenses, dust off with lens cleaning paper.  Moisten with a small amount of ethanol to remove sebum and other dirt.  

13. Diaphragm usage: Educational microscopes usually have a disc diaphragm and a disc with a large hole at the back of the platform.  Rotate it to select the hole to use and adjust the lighting range and Angle of incident light.  Even if the outside of the observed area is illuminated with a larger hole than necessary to brighten it, this is harmful and useless, for example by adding stray light.  Aperture is also related to the contrast of the image and the depth of focus (depth of focus), the smaller the aperture, the larger the aperture.  However, if the aperture is too small, the resolution will drop and darken, making it difficult to see.  

14. Focus adjustment: Preparations made from hand-sliced samples or water-sealed microorganisms are very thick and, especially at high magnification, impossible to focus on the entire surface at one time.  Therefore, the focus must always be observed when adjusting, but in this case, as it does not move much, a micro handle is used.  

15. Use of external light sources: When only reflectors are provided as lighting equipment, if natural light is used, it can only come from a certain direction inside the room.  It depends on the weather.  Thus, there is a light source device in which a straight fluorescent lamp is laid flat on a table and a microscope is arranged in front of the light source device.  There is also a simple light source that can be installed on the fixed parts instead of mirrors.  

optical microscope

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