Optical axis adjustment of a microscope

In the optical system of the microscope, the optical axis of the light source, condenser, objective and eyepiece, and the center of the aperture must be in line with the optical axis of the microscope, so the optical axis adjustment of a microscope must be adjusted before the microscopic examination, otherwise, the best observation effect cannot be achieved.

Microscopic organisms

Optical axis adjustment of a microscope

1. Light source filament adjustment

Old microscopes need to adjust the position of the light bulb. The light source of modern microscopes has been preset, so no adjustment is required.

2. Center adjustment of the condenser

In fact, the focus of optical axis adjustment of the microscope is the position adjustment of the condenser.

First view narrow aperture, with 10 x objective observation, visible in the field to view the outline of the diaphragm, if not in the central, two adjusting screws with the outside of the condenser will be transferred to the central part of its, then slowly increase the field of aperture, can see the beam to the field of peripheral evenly spread until the field of the diaphragm as fully and the field of edge inscribed, the outline of that already and shaft. And then slightly increase the field of view aperture, so that the contour image is just tangent to the field of view or slightly larger.

3. Adjustment of aperture apertures

Aperture installed in the condenser, the microscope on the outer edges of the condenser has a dial number and positioning, so easy to adjust the condenser and the numerical aperture of the objective to match, in principle, replacing the objective need to adjust the numerical aperture of the condenser, generally, the numerical aperture of the objective by 0.6 or 0.8 is the numerical aperture of the condenser.

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